DNA Barcoding Primer Mixes, 200 rxn

Ready-To-Use Primer Mixes for DNA Barcoding

£15.00 ex VAT

DNA Barcoding Primer Mixes, 200 rxn

Ready-To-Use Primer Mixes for DNA Barcoding

£15.00 ex VAT

For use with DNA Barcoding PCR Protocol.

 

Use these primer mixes to DNA barcode fungi, birds and mammals. The Ready-To-Use primer mix format is pre-prepared for easy use – just add the DNA template and master mix. Provided as a 10x concentrated stock solution, the 400 µL tube is suitable for up to 200 rxn.

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Description

Primers are short DNA sequences, which are used to define the region amplified during PCR. The Ready-To-Use primer mixes contain all the primers for a specific project, premixed and diluted to an easy-to-use concentration.

Details

  • Ready-To-Use: Skip rehydrating your primers, and just add DNA template and Master Mix.
  • Convenient: Save on time and pipette tips with a premixed solution
  • Easy: No need for dilution calculations with a 10X concentration – add 2uL to a 20µL reaction.

Applications

For use with DNA Barcoding PCR Protocol.

Use these primer mixes to DNA barcode the following:

  • fungi (ITS1F / ITS4)
  • birds (Bird F1 / Bird R1)
  • mammals (LCO1490 / HCO2198)

These primer mixes will allow you to amplify a segment of the internal transcribed spacer (ITS) region in fungi and a segment of the mitochondrial gene cytochrome c oxidase I (COI) gene in birds and mammals.

Specifications

Recommended Usage

Add 2 μL of primer mix per 20 µL PCR reaction. Add DNA template and PCR master mix as per the protocol, and top the reaction up with PCR grade water to a final volume of 20 µL.

Components

Primer Mix (10x stock solution)

Storage & Stability

Store dry at room temperature.

Shipping conditions

Shipped at room temperature.

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