Primers are short DNA sequences, which are used to define the region amplified during PCR. The Ready-To-Use primer mixes contain all the primers for a specific project, premixed and diluted to an easy-to-use concentration.
DNA Barcoding Primer Mixes, 200 rxn
Ready-To-Use Primer Mixes for DNA Barcoding
£15.95 ex VAT
DNA Barcoding Primer Mixes, 200 rxn
Ready-To-Use Primer Mixes for DNA Barcoding
£15.95 ex VAT
For use with DNA Barcoding PCR Protocol.
Use these primer mixes to DNA barcode fungi, birds, mammals, and plants. The Ready-To-Use primer mix format is pre-prepared for easy use – just add the DNA template and master mix. Provided as a 10x concentrated stock solution, the 400 µL tube is suitable for up to 200 rxn.
Description
Details
- Ready-To-Use: Skip rehydrating your primers, and just add DNA template and Master Mix.
- Convenient: Save on time and pipette tips with a premixed solution
- Easy: No need for dilution calculations with a 10X concentration – add 2uL to a 20µL reaction.
Applications
For use with DNA Barcoding PCR Protocol.
Use these primer mixes to DNA barcode the following:
- fungi (ITS1F / ITS4)
- birds (Bird F1 / Bird R1)
- mammals (LCO1490 / HCO2198)
These primer mixes will allow you to amplify a segment of the internal transcribed spacer (ITS) region in fungi and a segment of the mitochondrial gene cytochrome c oxidase I (COI) gene in birds and mammals.
Specifications
Recommended Usage
Add 2 μL of primer mix per 20 µL PCR reaction. Add DNA template and PCR master mix as per the protocol, and top the reaction up with PCR grade water to a final volume of 20 µL.
Components
Primer Mix (10x stock solution)
Storage & Stability
Store dry at room temperature.
Shipping conditions
Shipped at room temperature.
