A PCR master mix is a prepared mixture that contains all of the components for a PCR reaction that are not sample-specific. You just need to add a DNA template, primers and water.
5x HOT FIREPol® Blend Master Mix Ready to Load is an optimised ready-to-use PCR master mix for PCR assays requiring higher fidelity amplification and hot start functionality.
It contains a chemically deactivated FIREPol® DNA Polymerase that prevents the possibility of mispriming or primer dimer formation during room temperature setup. The polymerase is reactivated with a 12 – 15 min heating step as part of the initial denaturation step during PCR.
It also contains two carefully optimized enzymes: HOT FIREPol® DNA polymerase and a proofreading polymerase. The enzyme blend has 5’→ 3’ exonuclease activity and 3’→ 5’ proofreading activity for increased copy fidelity. This results in an increased fidelity (up to five fold) compared to regular Taq polymerase. Generated PCR products are compatible with blunt-end and T/A cloning procedures.
This master mix also contains dNTPs, MgCl2, BSA, and buffer.