|PM-ACT-400||400 µL (200 rxn)|
|PM-BIT-400||400 µL (200 rxn)|
|PM-GM-400||400 µL (200 rxn)|
|PM-LT-400||400 µL (200 rxn)|
|PM-MD-400||400 µL (200 rxn)|
|PM-RH-400||400 µL (200 rxn)|
For research and educational use only.
Primers are short DNA sequences, which are used to define the region amplified during PCR. The Ready-To-Use primer mixes contain all the primers for a specific project, premixed and diluted to an easy-to-use concentration. They are designed for the Bento DNA Analysis Projects, a range of hands-on projects exploring genetics.
For use with the Bento DNA Analysis Projects:
Storage & Stability
Store at 4ºC for up to nine months, or at –20 °C for longer term storage.
Temporary storage for up to 6 months at room temperature has no detrimental effects.
Shipped at room temperature.
Safety warnings and precautions
This product and its components are not considered hazardous in their given concentrations. However, as with all scientific reagents this product should be handled and stored with care as standard practice. Wear gloves. Care should be taken to avoid contact with skin or eyes. In case of contact with skin or eyes, wash immediately with water.
Quick Start Protocol
Label PCR tubes with a fine permanent marker, and make a list of samples and tube numbers.
For a 20 µL reaction:
- Pipette 2 µL of the Ready-To-Use Primer Mix into each PCR tube.
- Using new pipette tips each time, pipette DNA template and Master Mix into each tube.
- Make up the final volume by adding PCR Grade Water.
- Close the tubes, and mix well.
- Place in thermocycler, and run the appropriate PCR programme.
*per 20 µL reaction
*per 25 µL reaction
|Primer Mix||2 µL||2.5 µL||1.0 μM|
|PCR Master Mix||as per protocol||as per protocol||1X|
|DNA template||1-4 µL||1-5 µL||< 250ng|
|PCR Grade Water (up to final volume)||0 – 17 µL||0 – 22 µL|
|Total Volume||20 µL||25 µL|