Dipstick DNA Extraction Kitbeta

Go from sample to PCR in 30 seconds.

£27.50 ex VAT

Dipstick DNA Extraction Kitbeta

Go from sample to PCR in 30 seconds.

£27.50 ex VAT

Extract and clean genomic DNA with a simple 3 step protocol. The Dipstick DNA Extraction Kit is an equipment free protocol that goes from sample to DNA amplification in 30 seconds. Containing 100 dipsticks, and solutions of Extraction Buffer (50 mL) and Wash Buffer (100 mL), the kit includes enough reagents for up to 100 rxn.

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Description

The Dipstick DNA Extraction Kit is a really quick way to extract DNA and clean away contaminants. The resulting sample can be used for PCR assays, sequencing, and DNA barcoding. 

With low costs per sample, this method provides an affordable, reliable alternative to column-based extraction methods for very low DNA samples that require clean-up of inhibitors. This method is not suitable for protocols requiring total DNA extraction for genomic DNA. 

This protocol was published by Zou et al. (2017), when the group realised that cellulose-based paper allows rapid capture of DNA and a following wash step.

Details

  • Rapid: From sample to DNA amplification in 30 seconds.
  • Single wash step: remove remove PCR contaminants quickly 
  • Effective: obtain good yields with clean genomic DNA
  • Equipment-free: no need for columns, pipettes or centrifuges 
  • Easy workflow: A three steps method for gDNA extraction

Applications

Nucleic acid purification for use with PCR, quantitative real time PCR (qPCR) and the isothermal DNA amplification methods loop-mediated DNA amplification (LAMP) and recombinase polymerase amplification (RPA).

Specifications

Recommended Usage

Pipette 100 μL of Extraction Buffer into a 1.5 mL tube. Add 1-2 mm3 of a sample to the tube. Grind the sample with a pestle, and dilute the sample with an additional 400 μL of Extraction Buffer. Dip a dipstick up and down 3 times into the extract, then dip 5 times into 1 mL of wash buffer. Dip the dipstick into 20–50 µl DNA amplification reaction mix 3–15 times (~10 s total) to elute. 

Alternatively to store the sample, dip the dipstick into a tube of TE buffer. If necessary, molecular grade water can also be used for storage.

Limitations

The dipstick purification method does not significantly concentrate the DNA sample, unlike solid-phase nucleic acid techniques. This means the method is best suited to PCR-based applications, and not suitable for methods such as restriction enzyme digests or clean up of PCR amplicons.

Due to the small capture volume of the dipstick, this method is unsuitable for purifying large quantities of nucleic acids, which means it is not suited to methods such as genome sequencing that require larger quantities of DNA For multiple PCR reactions from the same sample, an individual dipstick will be required.

Components

Extraction Buffer (20 mM Tris-HCl, 25 mM NaCl, 2.5 mM EDTA, 0.05% SDS, 2% PVP-40, pH 8)

Wash Buffer (10 mM Tris-HCl, pH 8)

DNA Dipsticks (filterpaper, wax)

Storage & Stability

Store at room temperature (18–25 °C) for up to 1 year. For longer term storage, store at 4 ºC or freeze at –20 ºC.

Shipping conditions

Shipped at room temperature.

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