The HotSHOT DNA Extraction Kit provides users with a rapid, inexpensive, and reliable alkaline lysis method to extract PCR-quality DNA from a wide range of DNA-rich tissues. Suggested target tissues include animal tissues (from mammals to fish to invertebrates), although this protocol has also often been used successfully to extract fungal, plant, and bacterial DNA in a number of studies. It is a relatively crude DNA extraction method and is therefore not suitable for tissue types containing high proportions of PCR inhibitors.
This protocol was originally developed by Truett et al. (2000) for routine genotyping of mouse tissues. Since then it has been widely used in many different applications because of its low cost, simplicity, and scalability.