A PCR master mix is a pre-prepared mixture that contains all of the components for a PCR reaction that are not sample-specific. You just need to add a DNA template, primers and water.

5x FIREPol® Master Mix Ready to Load is an optimised ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq DNA polymerase FIREPol®, dNTPs, 7.5 mM MgCl₂, and loading dye, in a buffer.

• All-in-one format: reduces pipetting errors and saves time
• Convenient: ready to load directly onto gels after PCR – no need to add loading dye
• Stable: thermostable polymerase enzyme for room temperature set up, shipping, and short-term storage (up to 1 month unopened)
• Safe: contains no substances considered hazardous to health at their given concentrations
• Versatile: Suitable for templates up to 5 kb
• Affordable: suitable for routine work, and a cheaper alternative to lyophilised PCR beads

• Suitable for routine PCR
• Compatible with standard PCR instruments
• 5x FIREPol® Master Mix Ready to Load works great as an alternative to other commercial Taq Polymerase Master Mixes.
• As with all ready to load Master Mixes, it is not recommended for applications involving post-PCR spectrophotometric measurements (e.g. Sanger sequencing) without downstream dye cleanup, as loading dyes may interfere with measurements

Recommended usage

We recommend using 4 μL of 5x master mix per 20 μL reaction.

Components

• FIREPol^® DNA polymerase: highly processive, thermostable DNA polymerase
• 5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH₄)₂SO₄, 0.1% w/v Tween-20
• 7.5 mM MgCl₂: 1x PCR solution contains 1.5 mM MgCl₂
• 1 mM dNTPs of each: 1x PCR solution contains 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
• Blue dye: migration equivalent to 3.5–4.5 kb DNA fragment
• Yellow dye: migration equivalent to 35–45 bp DNA fragment
• Compound that increases sample density for direct loading

Storage & Stability

Routine storage at –20 ºC.

Temporary storage for up to 1 month at room temperature, or up to six months at 2 – 8 ºC, has no detrimental effects on the quality of 5x FIREPol^® Master Mix Ready To Load.

Shipping conditions

Shipped at room temperature.