Primers are short DNA sequences which are used to define the region amplified during PCR. The Ready-To-Use primer mixes contain all the primers for a specific project, premixed and diluted to an easy-to-use concentration.
Bird Sexing Primer Mixes, 200 rxn
Ready-To-Use Primer Mixes for Bird Sexing
£14.99 ex VAT
Bird Sexing Primer Mixes, 200 rxn
Ready-To-Use Primer Mixes for Bird Sexing
£14.99 ex VAT
Use these primer mixes with our Bird Sexing PCR Protocols to determine the sex of birds from DNA.
The mixes come in a convenient, ready-to-use 10× concentrated format – just add DNA and master mix. Each 400 µL tube provides enough mix for up to 200 PCR reactions.
Choose from four options. Each mix is compatible with a range of different bird species. Need help choosing the right for you? Explore our Bird Sexing Primer Selection Guide.
Description
Details
- Ready-To-Use: Skip rehydrating your primers, and just add DNA template and Master Mix.
- Convenient: Save on time and pipette tips with a premixed solution
- Easy: No need for dilution calculations with a 10X concentration – add 2uL to a 20µL reaction.
Applications
For use with the Bird Sexing Protocol.
Determine the sex of birds with these four different primer sets and DNA extracted from plucked feathers. This protocol allows you to test for the sex of a bird by amplifying a region of the CHD1-Z and CHD1-W genes on the Z and W sex chromosomes, using the feather calamus (the base of the feather) of plucked feathers as a DNA source.
Which primer set do I need?
To help you choose the most appropriate primer set for your bird species, we’ve created a detailed guide and searchable database of known results.
If you have any questions about which primers can be used with which bird species, please get in touch!
Specifications
Recommended Usage
Add between 2 μL of primer mix per 20 µL PCR reaction. Add DNA template and PCR master mix as per the protocol, and top the reaction up with PCR grade water to a final volume of 20 µL.
Components
Primer Mix (10x stock solution)
Storage & Stability
Unopened primers should be stable at room temperature for several months. Opened primers can be refrigerated at 4 °C between uses, but should be frozen at -20 °C to extend shelf life.
Shipping conditions
Shipped at room temperature.
