Primers are short DNA sequences which are used to define the region amplified during PCR. The Ready-To-Use primer mixes contain all the primers for a specific project, premixed and diluted to an easy-to-use concentration.

• Ready-To-Use: Skip rehydrating your primers, and just add DNA template and Master Mix.
• Convenient: Save on time and pipette tips with a premixed solution
• Easy: No need for dilution calculations with a 10X concentration – add 2uL to a 20µL reaction.

For use with the Bird Sexing Protocol.

Determine the sex of birds with these four different primer sets and DNA extracted from plucked feathers. This protocol allows you to test for the sex of a bird by amplifying a region of the CHD1-Z and CHD1-W genes on the Z and W sex chromosomes, using the feather calamus (the base of the feather) of plucked feathers as a DNA source.

Which primer set do I need?

To help you choose the most appropriate primer set for your bird species, we’ve created a detailed guide and searchable database of known results.

If you have any questions about which primers can be used with which bird species, please get in touch!

Recommended Usage

Add between 2 μL of primer mix per 20 µL PCR reaction. Add DNA template and PCR master mix as per the protocol, and top the reaction up with PCR grade water to a final volume of 20 µL.

Components

Primer Mix (10x stock solution)

Storage & Stability

Unopened primers should be stable at room temperature for several months. Opened primers can be refrigerated at 4 °C between uses, but should be frozen at -20 °C to extend shelf life.

Shipping conditions

Shipped at room temperature.