Primers are short DNA sequences, which are used to define the region amplified during PCR. The Ready-To-Use primer mixes contain all the primers for a specific project, premixed and diluted to an easy-to-use concentration.
Bird Sexing Primer Mixes, 200 rxn
Ready-To-Use Primer Mixes for Bird Sexing
£15.00 ex VAT
Bird Sexing Primer Mixes, 200 rxn
Ready-To-Use Primer Mixes for Bird Sexing
£15.00 ex VAT
For use with the Bird Sexing PCR Protocol.
Determine the sex of birds with these two different primer sets and DNA extracted from plucked feathers. The Ready-To-Use primer mix format is pre-prepared for easy use – just add the DNA template and master mix. Provided as a 10x concentrated stock solution, the 400 µL tube is suitable for up to 200 rxn.
Description
Details
- Ready-To-Use: Skip rehydrating your primers, and just add DNA template and Master Mix.
- Convenient: Save on time and pipette tips with a premixed solution
- Easy: No need for dilution calculations with a 10X concentration – add 2uL to a 20µL reaction.
Applications
For use with the Bird Sexing Protocol.
Determine the sex of birds with these two different primer sets and DNA extracted from plucked feathers. This protocol allows you to test for the sex of a bird by amplifying a region of the CHD1-Z and CHD1-W genes on the Z and W sex chromosomes, using the feather calamus (the base of the feather) of plucked feathers as a DNA source.
Which primer set do I need?
You can decide which primer set is most suitable based on what species you want to sex. Each primer set works for a range of species listed. This list is not exhaustive, and the primer sets may also have been shown to work for other species in scientific literature.
| Species | Scientific Order | Primer Set 1 (CHD1F/CHD1R) | Primer Set 2 (2550F/2718R) | Primer Set 3 (P0/P2/P8) |
|---|---|---|---|---|
| Landfowl, domestic chicken | Galliformes | ✓ | ✓ | |
| Pelican | Pelecaniformes | ✓ | ✓ | |
| Flamingo | Phoenicopteriformes | ✓ | ✓ | |
| Birds of prey, hawks, eagles, vultures | Accipitriformes | ✓ | ✓ | |
| Waterfowl | Anseriformes | ✓ | ✓ | |
| Songbirds | Passeriformes | ✓ | ||
| Stork, kestrel | Ciconiiformes | ✓ | ||
| Pigeons | Columbiformes | ✓ | ||
| Parrots | Psittaciformes | ✓ | ||
| Kookaburra | Coraciiformes | ✓ | ||
| Cranes, adzebills | Gruiformes |
Interested in a different species?
If you are looking to sex a species not listed above, please contact us.
Specifications
Recommended Usage
Add 2 μL of primer mix per 20 µL PCR reaction. Add DNA template and PCR master mix as per the protocol, and top the reaction up with PCR grade water to a final volume of 20 µL.
Components
Primer Mix (10x stock solution)
Storage & Stability
Store dry at room temperature.
Shipping conditions
Shipped at room temperature.
