Bird Sexing Bundlebeta

Everything you need to get started with bird sexing

£235.00 ex VAT

Bird Sexing Bundlebeta

Everything you need to get started with bird sexing

£235.00 ex VAT

Start processing samples with the bird sexing workflow. This bundle contains all the reagents, plastic consumables and accessories you need for the protocols DNA Extraction for Feathers and Bird Sexing PCR protocols. In addition to reusable materials, this kit contains enough reagents to process a minimum of 48 bird samples.


Note: the bundle does not include the device Bento Lab .



This bundle includes all the labware and consumables you need to follow the Bird Sexing Workflow, including the DNA Extraction from Feathers and Bird Sexing PCR protocols.

There is a choice of primer mix depending on the species of bird you are trying to sex. More details can be found on the product page for Bird Sexing Primer Mixes.

Note: The bundle does not include Bento Lab. To use this bundle, you will need access to an entry Bento Lab or similar equipment (i.e. a mini-centrifuge, a PCR thermocycler, a gel electrophoresis unit and a blue-light transilluminator).

You will also need a heat source to melt agarose for gel electrophoresis (e.g. a microwave, rice cooker or hot plate), and 1 L of distilled or deionised water to make up 0.5x TBE buffer from the 10X TBE buffer provided. Sterile distilled or deionised water can also be used for diluting DNA extractions prior to PCR. The protocols and reagent quantities are optimised for Bento Lab, but may be adapted.

Bundle content



Accessory Equipment

How many reactions can I do?

The bundle contains all of the starting materials that you will need to analyse bird DNA from feather samples.

You can analyse up to 48 samples with the materials included. Many of the reagents are included in excess, so you can restock key reagents to process more samples. The accessory equipment can be used for future work.

If you want to calculate the quantities and costs of reagents and consumables needed to sex larger numbers of birds, you can try our spreadsheet calculator here (Google account required). If you have any questions or feedback about the spreadsheet, please get in touch.

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  • Simple: Straightforward protocols to follow for DNA extraction, PCR and gel visualisation of results.
  • Scalable for low or high throughput: Suitable for a few samples or up to 30 samples per run. 
  • Rapid: Extraction of DNA in 30 minutes; PCR amplication and visualisation of DNA within 4 hours.
  • Affordable: Per reaction cost starting at £4 per sample; cost reduces with more samples.


This bundle contains all of the reagents, consumables and accessory materials for the Bird Sexing Workflow when used with equipment, such as Bento Lab.

Following this workflow, you can extract bird DNA from feathers or blood samples, and sex the bird using the molecular techniques of PCR and gel electrophoresis.

Which primer set do I need?

You can decide which primer set is most suitable based on what species you want to sex. Each primer set works for a range of species listed. This list is not exhaustive, and the primer sets may also have been shown to work for other species in scientific literature.

SpeciesScientific OrderPrimer Set 1 (CHD1F/CHD1R)Primer Set 2 (2550F/2718R)Primer Set 3 (P0/P2/P8)
Landfowl, domestic chicken Galliformes 
Birds of prey, hawks, eagles, vulturesAccipitriformes 
WaterfowlAnseriformes  ✓
Stork, kestrelCiconiiformes  
Cranes, adzebillsGruiformes  


Recommended Usage

This bundle is for use with the DNA Extraction from Feathers and the Bird Sexing PCR protocols.

Storage & Stability

HotSHOT DNA Extraction Kit should be stored sealed and in the original bag at room temperature or 4 ºC. Storage for up to 6 months at room temperature should have minimal detrimental effects provided the containers and bags are properly sealed. Exposure of the Alkaline Lysis Solution to air will gradually decrease the pH and effectiveness of this reagent over time. For longer term storage freeze at –20 ºC.

5X HOT FIREPol® Blend Master Mix, Bird Sexing Primer Mixes and 100 bp DNA Ladder should be stored in the freezer at –20 ºC and defrosted at 4 ºC before use.

All other components of the bundle can be permanently stored at room temperature.

Shipping conditions

Shipped at room temperature.