Bird Sexing – Materials Starter Kit

All the materials you need to get started with bird sexing

£235.00 ex VAT

Bird Sexing – Materials Starter Kit

All the materials you need to get started with bird sexing

£235.00 ex VAT

Start processing samples with the bird sexing workflow. This bundle contains all the reagents, plastic consumables and accessories you need for the protocols DNA Extraction for Feathers and Bird Sexing PCR protocols. In addition to reusable materials, this kit contains enough reagents to process a minimum of 48 bird samples.

 

Note: the bundle does not include equipment. You will need Bento Lab or an equivalent.

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Description

What’s included?

This bundle includes all the materials you need to use our guides to go from a bird sample (blood or feather) to a result using the Bird Sexing Workflow.

Chemical reagents

Plastic consumables

Accessory Equipment

What’s not included?

  • PCR equipment: To use these materials, you will need access to equipment, either a Bento Lab Entry or similar equipment (i.e. a mini-centrifuge, a PCR thermocycler, a gel electrophoresis unit and a blue-light transilluminator).
  • Microwave (or alternative): You will also need a heat source to melt agarose for gel electrophoresis (e.g. a microwave, rice cooker or hot plate)
  • Water: You will need 1L of distilled or deionised water to dilute the concentrated 10X TBE buffer provided in the bundle. This is easy and cheaper to find locally or on Amazon/eBay.

How many reactions can I do?

You can analyse up to 48 samples with the materials included, and the accessory equipment can be used for future work.

Many of the reagents are included in excess, so you can restock key reagents to process more samples. Email us if you would like advice to make the bundle suitable for a lager number of samples.

Want to explore sample cost for larger volumes?

If you want to calculate the quantities and costs of reagents and consumables needed to sex larger numbers of birds, you can try our spreadsheet calculator here. If you have any questions or feedback about the spreadsheet, please get in touch.

Details

  • Simple: Straightforward protocols to follow for DNA extraction, PCR and gel visualisation of results.
  • Scalable for low or high throughput: Suitable for a few samples or up to 30 samples per run. 
  • Rapid: Extraction of DNA in 30 minutes; PCR amplication and visualisation of DNA within 4 hours.
  • Affordable: Per reaction cost starting at £4 per sample; cost reduces with more samples.

Applications

This bundle contains all of the reagents, consumables and accessory materials for the Bird Sexing Workflow when used with equipment, such as Bento Lab.

Following this workflow, you can extract bird DNA from feathers and sex the bird using the molecular techniques of PCR and gel electrophoresis.

Specifications

Recommended Usage

This bundle is for use with the DNA Extraction from Feathers and the Bird Sexing PCR protocols.

Storage & Stability

HotSHOT DNA Extraction Kit should be stored sealed and in the original bag at room temperature or 4 ºC. Storage for up to 6 months at room temperature should have minimal detrimental effects provided the containers and bags are properly sealed. Exposure of the Alkaline Lysis Solution to air will gradually decrease the pH and effectiveness of this reagent over time. For longer term storage freeze at –20 ºC.

5X HOT FIREPol® Blend Master Mix, Bird Sexing Primer Mixes and 100 bp DNA Ladder should be stored in the freezer at –20 ºC and defrosted at 4 ºC before use.

All other components of the bundle can be permanently stored at room temperature.

Shipping conditions

Shipped at room temperature.

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